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KMID : 0368420120550040298
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Journal of Plant Biology
2012 Volume.55 No. 4 p.298 ~ p.302
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The acetylation level of rDNA in Brassica campestris
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Qiu Zhengming
Zhang Lu
Hu Yong
He Shibin
Li Lijia
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Abstract
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Ribosomal DNA is an important repeated sequence that forms the nucleolus at the interphase. Its transcription into ribosomal RNA for ribosome biogenesis also represents a transitional point for several cellular processes, including cell-cycle progression, gene-silencing, and formation of the ribonucleoprotein complex. The levels of rDNA acetylation have an important role in regulating structural changes in rDNA chromatin and transcriptional activity. Using root-tip samples from Brassica campestris, we determined that some rDNA chromatin is located in the heterochromatin regions while some is de-condensed and found in euchromatin regions. Immuno-staining results showed that histone H4K5 acetylation and H4 tetra-acetylation signals are dispersed within the euchromatin. Analysis of the promoter and exon regions of rDNA via chromatin immuno-precipitation (ChIP) revealed a connection between histone acetylation and rDNA conformation.
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KEYWORD
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Brassica campestris, Chromatin de-condensation, Heterochromatin, Histone acetylation, Ribosomal DNA
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